對于很多科(ke)研(yan)汪(wang)來說，細(xi)(xi)(xi)胞(bao)(bao)是脆弱(ruo)又重(zhong)要的(de)(de)寶貝之一，承擔著(zhu)重(zhong)要的(de)(de)實(shi)驗數據希望(wang)。尤(you)其(qi)是在凍(dong)(dong)存(cun)復蘇的(de)(de)時(shi)候，經常會因為(wei)一些小細(xi)(xi)(xi)節導致(zhi)問題。比如：沒有控(kong)制(zhi)好(hao)細(xi)(xi)(xi)胞(bao)(bao)的(de)(de)生(sheng)(sheng)(sheng)長(chang)密(mi)(mi)度(du)細(xi)(xi)(xi)胞(bao)(bao)的(de)(de)密(mi)(mi)度(du)對細(xi)(xi)(xi)胞(bao)(bao)的(de)(de)生(sheng)(sheng)(sheng)存(cun)質量(liang)有著(zhu)重(zhong)要的(de)(de)影響(xiang)，畢竟它們是一群又不能擠著(zhu)了(le)也不能孤獨的(de)(de)嬌貴寶寶。密(mi)(mi)度(du)過高(gao)會讓細(xi)(xi)(xi)胞(bao)(bao)沒有足夠的(de)(de)生(sheng)(sheng)(sheng)存(cun)空間，密(mi)(mi)度(du)過低(di)會讓細(xi)(xi)(xi)胞(bao)(bao)無(wu)法互(hu)相連接健康(kang)生(sheng)(sheng)(sheng)長(chang)。建議大(da)家在細(xi)(xi)(xi)胞(bao)(bao)接種前，一定要調(diao)整好(hao)適合細(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)長(chang)的(de)(de)濃(nong)度(du)，提高(gao)細(xi)(xi)(xi)胞(bao)(bao)的(de)(de)存(cun)活率。溫度(du)控(kong)制(zhi)不達標慢凍(dong)(dong)快融是細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)復蘇的(de)(de)*關鍵詞(ci)之一存(cun)細(xi)(xi)(xi)胞(bao)(bao)時(shi)需現配凍(dong)(dong)存(cun)液(如購買(mai)市面上通用的(de)(de)含(han)血(xue)(xue)清細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)液。杭州長(chang)沙無(wu)血(xue)(xue)清細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)液

無(wu)血(xue)(xue)(xue)清(qing)細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液，含(han)多種保(bao)護(hu)(hu)劑成(cheng)(cheng)分(fen)。一些保(bao)護(hu)(hu)劑，易于穿透細(xi)(xi)胞(bao)(bao)(bao)，避免細(xi)(xi)胞(bao)(bao)(bao)內(nei)部水分(fen)子(zi)形成(cheng)(cheng)冰晶損(sun)傷細(xi)(xi)胞(bao)(bao)(bao)，同時另一些保(bao)護(hu)(hu)劑不能穿透細(xi)(xi)胞(bao)(bao)(bao)，但可(ke)以在冰晶形成(cheng)(cheng)之前，優(you)先結合細(xi)(xi)胞(bao)(bao)(bao)外的水分(fen)子(zi)，降(jiang)低細(xi)(xi)胞(bao)(bao)(bao)外溶液的電解質濃(nong)度，減(jian)少陽(yang)離(li)子(zi)進入細(xi)(xi)胞(bao)(bao)(bao)的數量，為多種保(bao)護(hu)(hu)劑組合，在細(xi)(xi)胞(bao)(bao)(bao)內(nei)外進行(xing)雙重(zhong)保(bao)護(hu)(hu)，較大提高了細(xi)(xi)胞(bao)(bao)(bao)復(fu)蘇存(cun)(cun)活率。無(wu)血(xue)(xue)(xue)清(qing)細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液不含(han)牛血(xue)(xue)(xue)清(qing)，無(wu)動物源組份。2-8℃即可(ke)穩定保(bao)存(cun)(cun)，無(wu)需(xu)冷凍(dong)(dong)，即取即用。凍(dong)(dong)存(cun)(cun)細(xi)(xi)胞(bao)(bao)(bao)，無(wu)需(xu)程序降(jiang)溫(wen)。凍(dong)(dong)存(cun)(cun)細(xi)(xi)胞(bao)(bao)(bao)復(fu)蘇率在90%以上。重(zhong)慶正規無(wu)血(xue)(xue)(xue)清(qing)細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液廠家批發價無(wu)血(xue)(xue)(xue)清(qing)細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液使用方法(fa)：加入適量的無(wu)血(xue)(xue)(xue)清(qing)型細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液于離(li)心管中。

無(wu)血清(qing)細(xi)胞(bao)(bao)(bao)(bao)(bao)凍存(cun)液的(de)操作(zuo)規范：1、培養(yang)細(xi)胞(bao)(bao)(bao)(bao)(bao)至(zhi)對數(shu)生長(chang)晚期，顯微鏡(jing)觀察其外觀、形態(tai)(tai)、有(you)無(wu)污染等。選擇狀(zhuang)態(tai)(tai)良(liang)好(hao)的(de)細(xi)胞(bao)(bao)(bao)(bao)(bao)進行凍存(cun)操作(zuo)(注：貼壁生長(chang)細(xi)胞(bao)(bao)(bao)(bao)(bao)，用胰蛋(dan)白酶消(xiao)化(hua)并用完全培養(yang)基(ji)終(zhong)止消(xiao)化(hua)，進行細(xi)胞(bao)(bao)(bao)(bao)(bao)計(ji)數(shu);懸浮生長(chang)細(xi)胞(bao)(bao)(bao)(bao)(bao)，進行細(xi)胞(bao)(bao)(bao)(bao)(bao)計(ji)數(shu))。2、500～1000g離(li)心5min，棄(qi)上清(qing)。3、加(jia)入適量的(de)細(xi)胞(bao)(bao)(bao)(bao)(bao)凍存(cun)液，重(zhong)懸細(xi)胞(bao)(bao)(bao)(bao)(bao)，使(shi)細(xi)胞(bao)(bao)(bao)(bao)(bao)濃(nong)度達到(dao)1～10×106/ml，置于凍存(cun)管中密閉。4、采取(qu)逐級降溫保存(cun)：4℃放(fang)(fang)置20min，-20℃放(fang)(fang)置30min，-70℃過(guo)夜，置于液氮罐中長(chang)期存(cun)儲。注意：務必超(chao)凈工作(zuo)臺內無(wu)菌操作(zuo)，避免污染。雜交(jiao)瘤細(xi)胞(bao)(bao)(bao)(bao)(bao)凍存(cun)時應定期復蘇，以(yi)檢查細(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)活性(xing)和分泌(mi)抗體的(de)穩定性(xing)。

無血(xue)(xue)清(qing)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)液(ye)通用于各(ge)種動(dong)(dong)物(wu)細(xi)(xi)胞(bao)(bao)株(zhu)（tumour細(xi)(xi)胞(bao)(bao)和(he)常(chang)規細(xi)(xi)胞(bao)(bao)），大(da)部(bu)分(fen)的(de)干細(xi)(xi)胞(bao)(bao)，凍(dong)(dong)(dong)存(cun)細(xi)(xi)胞(bao)(bao)可在-80℃長期(qi)(qi)(qi)保(bao)存(cun)。不含動(dong)(dong)物(wu)源性蛋(dan)白，不含血(xue)(xue)清(qing)成(cheng)分(fen)，可有效減少各(ge)類細(xi)(xi)菌(jun)、病毒和(he)支原體等污染，保(bao)證凍(dong)(dong)(dong)存(cun)細(xi)(xi)胞(bao)(bao)的(de)安全。該凍(dong)(dong)(dong)存(cun)液(ye)含DMSO、葡(pu)萄糖等各(ge)種細(xi)(xi)胞(bao)(bao)營(ying)養成(cheng)分(fen)，提高(gao)細(xi)(xi)胞(bao)(bao)存(cun)活率和(he)活力，亦適合于無血(xue)(xue)清(qing)培養細(xi)(xi)胞(bao)(bao)和(he)蛋(dan)白表達細(xi)(xi)胞(bao)(bao)。產品優(you)勢：1、無需(xu)程序性降溫，可直(zhi)接(jie)將細(xi)(xi)胞(bao)(bao)置于-80℃凍(dong)(dong)(dong)存(cun)，凍(dong)(dong)(dong)存(cun)液(ye)無需(xu)稀(xi)釋(shi)。2、細(xi)(xi)胞(bao)(bao)可于-80℃長期(qi)(qi)(qi)保(bao)存(cun)。3、凍(dong)(dong)(dong)存(cun)液(ye)不含血(xue)(xue)清(qing)等，較大(da)降低(di)細(xi)(xi)胞(bao)(bao)污染的(de)可能(neng)性。保(bao)存(cun)方法：冰(bing)袋運(yun)輸，4℃保(bao)存(cun)，保(bao)質期(qi)(qi)(qi)一年。無血(xue)(xue)清(qing)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)液(ye)可用于其他類型哺乳(ru)動(dong)(dong)物(wu)細(xi)(xi)胞(bao)(bao)的(de)儲存(cun)。

無(wu)(wu)血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液(ye)的使(shi)(shi)用(yong)(yong)方法(fa)及(ji)注意事項：無(wu)(wu)血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液(ye)：含多種(zhong)保(bao)護(hu)劑(ji)成分(fen),一些(xie)保(bao)護(hu)劑(ji),易于(yu)穿透細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao),避(bi)免(mian)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)內(nei)(nei)部水(shui)(shui)分(fen)子(zi)(zi)形(xing)成冰(bing)晶損傷細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao),同(tong)時另一些(xie)保(bao)護(hu)劑(ji)不能穿透細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao),但可以在(zai)冰(bing)晶形(xing)成之前(qian),優先結合細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)外的水(shui)(shui)分(fen)子(zi)(zi),降低細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)外溶液(ye)的電(dian)解(jie)質濃度,減少(shao)陽離子(zi)(zi)進入(ru)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的數(shu)量,我公司無(wu)(wu)血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液(ye),為多種(zhong)保(bao)護(hu)劑(ji)組合,在(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)內(nei)(nei)外進行雙重(zhong)保(bao)護(hu),較大(da)提高了細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)復蘇存(cun)(cun)活率。【產品用(yong)(yong)途】1.用(yong)(yong)于(yu)免(mian)疫細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)及(ji)干細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的存(cun)(cun)儲。2.細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)保(bao)藏中(zhong)心(xin)，用(yong)(yong)于(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株的長期(qi)保(bao)存(cun)(cun)。3.科研(yan)高校，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)株凍(dong)(dong)存(cun)(cun)。4.醫院(yuan)樣(yang)本庫細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)樣(yang)本保(bao)存(cun)(cun)。【使(shi)(shi)用(yong)(yong)方法(fa)】1、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)前(qian)準(zhun)備（1）要(yao)(yao)求凍(dong)(dong)存(cun)(cun)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)在(zai)對數(shu)生長期(qi)，且活率大(da)于(yu)90%。（2）計(ji)算細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)密度，一般(ban)要(yao)(yao)求密度在(zai)1-3x10cells/mL，不同(tong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)系請參照附表(biao)。2、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)過程（1）將要(yao)(yao)凍(dong)(dong)存(cun)(cun)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)懸液(ye)，進行細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)計(ji)數(shu)，計(ji)數(shu)后(hou)(hou)離心(xin)，8003min即可。度保(bao)存(cun)(cun)的無(wu)(wu)血(xue)(xue)清(qing)凍(dong)(dong)存(cun)(cun)液(ye)緩慢(man)加入(ru)離心(xin)后(hou)(hou)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)沉淀中(zhong)，根據密度調整(zheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液(ye)用(yong)(yong)量。（3）反復吹吸混勻(yun)后(hou)(hou)，分(fen)裝于(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)管(guan)中(zhong)，每(mei)管(guan)500ul-1000ul，（建議500ul/管(guan)）。無(wu)(wu)血(xue)(xue)清(qing)凍(dong)(dong)存(cun)(cun)液(ye)使(shi)(shi)用(yong)(yong)方法(fa)：將凍(dong)(dong)存(cun)(cun)管(guan)直接轉移至-80C水(shui)(shui)箱中(zhong)冷凍(dong)(dong)保(bao)存(cun)(cun)。廣州(zhou)正規無(wu)(wu)血(xue)(xue)清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)液(ye)廠家直銷

可以防止或減少冷凍冰(bing)晶對細(xi)胞的損(sun)傷作用(yong)。杭州長(chang)沙無血(xue)清細(xi)胞凍存液

無(wu)(wu)血清(qing)快速細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)，通用(yong)于(yu)各(ge)種(zhong)動物(wu)(wu)細(xi)(xi)胞(bao)(bao)株。特別配(pei)方具(ju)有(you)有(you)效提高(gao)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)活率和(he)(he)復蘇活力(li)。不(bu)含動物(wu)(wu)來源性(xing)蛋(dan)白(bai)，能減(jian)少各(ge)類病毒、霉菌和(he)(he)支原體等的(de)(de)污染(ran)，確(que)保凍(dong)(dong)(dong)存(cun)(cun)(cun)細(xi)(xi)胞(bao)(bao)安全(quan)(quan)。既(ji)適用(yong)于(yu)一(yi)般培養細(xi)(xi)胞(bao)(bao)的(de)(de)凍(dong)(dong)(dong)存(cun)(cun)(cun)，也適用(yong)于(yu)無(wu)(wu)血清(qing)培養細(xi)(xi)胞(bao)(bao)和(he)(he)蛋(dan)白(bai)表達細(xi)(xi)胞(bao)(bao)的(de)(de)凍(dong)(dong)(dong)存(cun)(cun)(cun)。產(chan)品(pin)(pin)特色：高(gao)安全(quan)(quan)性(xing)，完全(quan)(quan)使用(yong)醫(yi)藥品(pin)(pin)等級原料進行生產(chan)，不(bu)含動物(wu)(wu)成分，病毒、霉菌和(he)(he)支原體等污染(ran)可能性(xing)低，各(ge)批(pi)產(chan)品(pin)(pin)之間有(you)更高(gao)的(de)(de)產(chan)品(pin)(pin)質量一(yi)致性(xing)。細(xi)(xi)胞(bao)(bao)存(cun)(cun)(cun)活率高(gao)，無(wu)(wu)批(pi)次差異。完全(quan)(quan)凍(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)配(pei)方，可直(zhi)(zhi)接(jie)使用(yong)，方便簡(jian)捷(jie)，可直(zhi)(zhi)接(jie)存(cun)(cun)(cun)放于(yu)-70℃冰箱(xiang)凍(dong)(dong)(dong)存(cun)(cun)(cun)，無(wu)(wu)需經(jing)過費時(shi)的(de)(de)程(cheng)序降溫過程(cheng)（省(sheng)(sheng)時(shi)、省(sheng)(sheng)力(li)、省(sheng)(sheng)錢）。杭(hang)州長沙無(wu)(wu)血清(qing)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)

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